AMMONIUM DEHYDROGENASE

Determination of Ammonium Ion Using a Reagentless Amperometric Biosensor Based on Immobilized Alanine Dehydrogenase

The use of the enzyme alanine dehydrogenase (AlaDH) for the determination of ammonium ion (NH(4)(+)) usually requires the addition of pyruvate substrate and reduced nicotinamide adenine dinucleotide (NADH) simultaneously to effect the reaction. This addition of reagents is inconvenient when an enzyme biosensor based on AlaDH is used. To resolve the problem, a novel reagentless amperometric bios...

Evidence for Ammonium-dependent de Novo Synthesis of Glutamate Dehydrogenase in Detached Oat Leaves.

Glutamate dehydrogenase becomes density labeled through the incorporation of deuterium and (15)N when detached oat leaves (Avena sativa var. Fulghum) are incubated in the presence of ammonia. The enzyme has been isolated by means of DEAE-cellulose chromatography, ammonium sulfate precipitation, isopycnic equilibrium centrifugation, and disc electrophoresis from leaves fed l-methionine-(35)S. Ra...

Ammonium benzene­phospho­nate

In the crystal structure of the title salt, NH(4) (+).[(C(6)H(5))P(O)(2)(OH)](-) or NH(4) (+)·C(6)H(6)O(3)P(-), the N and O atoms inter-act via hydrogen bonds to generate a layer motif. The phenyl rings are stacked above and below this layer, sandwiching the hydrogen-bonded layer.

Substrate specificity of Rhbg: ammonium and methyl ammonium transport.

Rhbg is a nonerythroid membrane glycoprotein belonging to the Rh antigen family. In the kidney, Rhbg is expressed at the basolateral membrane of intercalated cells of the distal nephron and is involved in NH4+ transport. We investigated the substrate specificity of Rhbg by comparing transport of NH3/NH4+ with that of methyl amine (hydrochloride) (MA/MA+), often used to replace NH3/NH4+, in oocy...

Ammonium Perchlorate and Ammonium Perchlorate - Binder Sandwich Combustion